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KMID : 0356420020200020087
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Journal of Korean Andrology
2002 Volume.20 No. 2 p.87 ~ p.93
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Antioxidative Effect of Rebamipide in Cryopreservation and Thawing of Sperm
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Park Nam-Cheol
Park Eun
Lee Kyong-Mi
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Abstract
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Purpose: We performed this study to investigate the effects of adding rebamipide to cryoprotectant, to identify the improvement in sperm parameters by antioxidative action.
Materials and Methods: Semen samples from 30 normal healthy volunteers were collected. Rebapimide was added with various concentration in cryoprotectant. All specimens were cryopreservated for 3 days and thawed at room temperature. Sperm motility, vitality, fertile activity, levels of ROS and lipid peroxidation were analyzed by semen analysis, eosin-nigrosin stain, hypo-osmotic swelling test, chemiluminescence and thiobarbituric acid method, respectively, before and after cryopreservation and thawing.
Results: After cryopreservation and thawing, the sperm motility was significantly decreased in all concentrations, but motility was higher in proportion to concentration of rebapimide(p＜0.05). The sperm vitality showed no significant difference related to the concentration of rebamipide related cryopreservation(p＞0.05). The levels of seminal ROS and the lipid peroxidation were significantly decreased in proportion to the concentration of rebamipide after cryopreservation and thawing(p＜0.05), but the total swelling rate in hypo-osmotic swelling test was significantly increased(p＜0.05).
Conclusions: These results suggest that rebamipide is an effective free radical scavenger to protect sperm from oxidative cellualr damage during cryopreservation and thawing.
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KEYWORD
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Male infertility, Reactive oxygen species, Rebamipide, Cryopreservation
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